Coding

Part:BBa_K4390067

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-03)


SpyCatcher C part

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a Level 0 part of type C part generating the following 4 base overhangs at upstream (TTCG) and downstream (GCTT) ends.

Usage and Biology

This is a C part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None